Journal: International Journal of Nanomedicine

Article Title: Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors

doi: 10.2147/IJN.S197990

Figure Lengend Snippet: Evaluation of Mal-HSA-FITC uptake by different cell types. Fluorescence microscopy images of Mal-HSA-FITC probes (green) incubated for 3 h with ( A ) THP-1 macrophages, ( C ) HUVECs (EC), and ( D ) HCtSMCs (SMC). ( B ) Mal-HSA-FITC uptake by different cell types plotted against time. The results represent data from technical triplicates (9 images/well and timepoint) and are presented as mean and SEM. Data show significantly increased uptake of modified HSA probes by THP-1 cells compared with HUVEC (**** P <0.0001) and HCtSMCs ( #### P<0.0001) at 2.5 h and 3 h. None of the cell types recognized HSA-FITC (data not shown) (repeated measures two-way ANOVA (factors: cell type and time) and Tukey’s multiple comparison test). Abbreviations: HSA, human serum albumin; GCU, green calibration unit.

Article Snippet: Human carotid smooth muscle cells (HCtSMCs) (Cell Applications, San Diego, CA, USA) were cultured up to passage 7 in human smooth muscle cell media (Cell Applications).

Techniques: Fluorescence, Microscopy, Incubation, Modification, Comparison

Journal: PloS one

Article Title: Embedding synthetic microvascular networks in poly(lactic acid) substrates with rounded cross-sections for cell culture applications.

doi: 10.1371/journal.pone.0073188

Figure Lengend Snippet: Figure 3. Seeding and culture of bovine aortic endothelial cells (BAECs) throughout PLA microchannel networks. (a) Confocal microscopy shows that the interior walls of a 200 mm diameter straight circularized microchannel can be uniformly seeded with endothelial cells that subsequently are confluently cultured in a monolayer lining the channel wall. (b) Fluorescent images show BAECs survive and maintain their morphology after 5 days in the straight circularized microchannel (bar, 50 mm). (c) BAECs seeded in four generations of branched microchannel network with diameters extending below 50 mm uniformly cover all channel walls and maintain viability after 3 days of culture (bar, 50 mm). doi:10.1371/journal.pone.0073188.g003

Article Snippet: Bovine Aortic Endothelial Cells (BAECs; Cell Applications, Inc., CA) obtained as a kind gift from Dr. Mariah Hahn at Texas A&M University were resuspended at 56106 cells mL–1 medium for 500 mm diameter channels or at 26107 cells mL–1 medium for 100 mm diameter and branched channels.

Techniques: Confocal Microscopy, Cell Culture